Methodological peculiarities of basal respiration measurement of organogenic samples of peat soils

Abstract

Methodological peculiarities of basal respiration (BR) measurement have been studied on the peat soil samples from two taiga subzones. It is found that the BR values at the initial incubation time (30 minutes) significantly exceed the ‘classical’ variant (24 hours) and gradually decrease with time. It is observed for all types of samples. The dependence of BR on the volume of the gas phase in the system was revealed - the values are higher the larger the volume of the incubation vial and the larger the ratio of the volume of the gas phase: mass (volume) of the sample. Initial values of CO2 concentration do not influence the dynamics of BR, because both at about zero and relatively high (4400 ppm) starting values of CO2 content the results of incubation were similar. It is critical to maintain the same incubation time interval, the same vial and sample volume while assessing the biological activity of soils using the BR method. The standard variant of BR determination during 24-hour incubation showed results that differed minimally depending on vial volumes and is probably preferable in this variant of experiments.

References

1. Ананьева Н.Д., Сусьян Е.А., Гавриленко Е.Г. Особенности определения углерода микробной биомассы почвы методом субстрат-индуцированного дыхания // Почвоведение. 2011. № 11. С. 1327–1333. 2. Благодатская Е.В., Ананьева Н.Д., Мякшина Т.Н. Характеристика состояния микробного сообщества по величине метаболического коэффициента // Почвоведение. 1995. № 2. С. 205–210. 3. Вадюнина А.Ф., Корчагина З.А. Методы исследования физических свойств почв. М., 1986. 416 с. 4. Воробьева Л.А. Теория и практика химического анализа почв. М., 2006. 400 с. 5. Гончарова О.Ю., Матышак Г.В., Бобрик А.А. и др. Оценка вклада корневого и микробного дыхания в общий поток СО2 из торфяных почв и подзолов севера Западной Сибири методом интеграции компонентов // Почвоведение. 2019. № 2. С. 234–245. 6. Евдокимов И.В. Методы определения биомассы почвенных микроорганизмов // RJEE. 2018. Т. 3, № 3. https://doi.org/10.21685/2500-0578-2018-3-5 7. Ковалева Е.И., Трофимов С.Я., Шоба С.А. Функционирование почв в условиях нефтяного загрязнения в таежной зоне Западной Сибири // Вестн. Моск. ун-та. Сер. 17. Почвоведение. 2024. № 4. С. 214–227. 8. Маслов М.Н., Маслова О.А., Токарева О.А. Изменение лабильного и микробного пулов углерода и азота в лесной подстилке при разных способах хранения образцов // Почвоведение. 2019. № 7. С. 793–802. 9. Матышак Г.В., Богатырев Л.Г., Гончарова О.Ю. и др. Особенности развития почв гидроморфных экосистем северной тайги Западной Сибири в условиях криогенеза // Почвоведение. 2017. № 10. С. 1155–1164. 10. Смагин А.В. Газовая фаза почв. М., 2005. 301 с. 11. Смагин А.В., Садовникова Н.Б., Щерба Т.Э., Шнырев Н.А. Абиотические факторы дыхания почв // Экологический вестн. Северного Кавказа. 2009. Т. 6, № 1. С. 5–19. 12. Шишов Л.Л., Тонконогов В.Д., Лебедева И.И., Герасимова М.И. Классификация и диагностика почв России. Смоленск, 2004. 341 с. 13. Anderson J.P.E., Domsch K.H. A physiological method for the quantitative measurement of microbial biomass in soils // Soil Biol. Biochem. 1978. Vol. 10, № 3. P. 215–221. https://doi.org/10.1016/0038-0717(78)90099-8 14. Bekku Y., Koizumi H., Oikawa T. et al. Examination of four methods for measuring soil respiration // Appl. Soil Ecol. 1997. Vol. 5, № 3. https://doi.org/10.1016/S0929-1393(96)00131-X 15. Chen X., Tang J., Jiang L. et al. Evaluating the impacts of incubation procedures on estimated Q10 values of soil respiration // Soil Biol. Biochem. 2010. Vol. 42, № 12. P. 2282–2288. https://doi.org/10.1016/j.soilbio.2010.08.030 16. Creamer R.E., Schulte R.P.O., Stone D. et al. Measuring basal soil respiration across Europe: Do incubation temperature and incubation period matter? // Ecol. Indic. 2014. Vol. 36. https://doi.org/10.1016/j.ecolind.2013.08.015 17. Guntiñas M.E., Gil-Sotres F., Leiros M.C. et al. Sensitivity of soil respiration to moisture and temperature // J. Soil Sci. Plant Nutr. 2013. Vol. 13, № 2. http://dx.doi.org/10.4067/S0718-95162013005000035 18. Hofman J., Dušek L., Klánová J. et al. Monitoring microbial biomass and respiration in different soils from the Czech Republic – a summary of results // Environ. Int. 2004. Vol. 30, № 1. P. 19–30. https://doi.org/10.1016/S0160-4120(03)00142-9 19. ISO 16072 (2002). Soil quality – laboratory methods for determination of microbial soil respiration. 20. ISO 17155 (2003). Soil quality – Determination of abundance and activity of the soil microflora using respiration curves. 21. IUSS Working Group WRB. World Reference Base for Soil Resources. International soil classification system for naming soils and creating legends for soil maps. 4th edition. International Union of Soil Sciences (IUSS), Vienna, Austria, 2022. 22. Koizumi H., Nakadai T., Usami Y. et al. Effect of carbon dioxide concentration on microbial respiration in soil // Ecol. Res. 1991. Vol. 6. P. 227–232. 23. Meyer N., Welp G., Amelung W. Effect of sieving and sample storage on soil respiration and its temperature sensitivity (Q10) in mineral soils from Germany // Biol. Fertil. Soils. 2019. Vol. 55, № 8. P. 825–832. https://doi.org/10.1007/s00374-019-01374-7 24. Pitombo L.M., Ramos J.C., Quevedo H.D. et al. Methodology for soil respirometric assays: Step-by-step and guidelines to measure fluxes of trace gases using microcosms// MethodsX. 2018. Vol. 5. P. 656–668. 25. Ritz K., Harris J.A., Pawlett M. et al. Using Science to Create a Better Place: Catabolic Profiles as an Indicator of Soil Microbial Functional Diversity // Environment Agency Science Report. SC040063/SR. 2006. 26. Stanek W., Silc T. Comparisons of four methods for determination of degree of peat humification (decomposition) with emphasis on the von Post method // Can. J. Soil Sci. 1977. Vol. 57, № 2. P. 109–117. https://doi.org/10.4141/cjss77-015 27. Uzun N., Uyanoz R. Determination of urease catalase activities and CO2 respiration in different soils obtained from in semi-arid region Konya, Turkey // Trends in Soil & Plant Sciences Journal. 2011. Vol. 2. P. 1–6. 28. Vanhala P., Tamminen P., Fritze H. Relationship between basal soil respiration rate, tree stand and soil characteristics in boreal forests // Environ. Monit. Assess. 2005. Vol. 101. P. 85–92. https://doi.org/10.1007/s10661-005-9134-0 29. Wade J., Culman S.W., Hurisso T.T. et al. Sources of variability that compromise mineralizable carbon as a soil health indicator // Soil Sci. Soc. Am. J. 2018. Vol. 82, № 1. P. 243–252. https://doi.org/10.2136/sssaj2017.03.0105 30. Zhou W., Hui D., Shen W. Effects of soil moisture on the temperature sensitivity of soil heterotrophic respiration: a laboratory incubation study // PloS one. 2014. Vol. 9, № 3. https://doi.org/10.1371/journal.pone.0092531